ZEIN
Categories | Plant Extracts and Flavours |
---|---|
light yellow | powder |
Update Time | Mar 29,2024 |
ZEIN, REGULAR GRADE
FOR PHARMACEUTICAL, COSMETIC AND FOOD USE
ZEIN is the water-insoluble prolamine protein of corn gluten.
Description: | Straw-to-yellow colored granular powder, bland in taste. |
Approximate Molecular Weight: | 35,000 |
Bulk Density Range: | 1.25 - 2.1 gm/10ml |
Identification Tests: | Positive for (A) and (B) conforms to solubility tests below. |
Solubility in Water: | Insoluble |
Solubility in 80% alcohol: | Clear to slightly cloudy |
ZEIN (Protein): | 88-96% calculated on a dry basis |
Loss on Drying: | 8% maximum (drying for 2 hours at 105 degrees C) |
Total Ash: | 2% maximum |
Heavy Metals: | 20 ppm maximum |
Arsenic: | 3 ppm maximum |
Mesh Size: | 100% through U.S. 20 mesh |
Microbial Limits: | The total bacterial count does not exceed 1000 per gram and the tests for Salmonella species and Escherichia coli are negative. |
Standard Packaging: | 25 kilo poly bag inside double-walled corrugated cartons (55.12 lbs. net) |
ASSAY METHODS FOR ZEIN
Identification Tests
1. Add a few drops of nitric acid t.s. to an aqueous ZEIN suspension - heat it. The solution will be a light yellow color. After adding ammonia t.s., an orange color develops.
2. Add a few drops of copper sulfate t.s. to an alkaline ZEIN solution and warm it in a warm bath. A purple color will develop.
Assay
Proceed as directed under “Nitrogen Determination” (461) USP XXI, p. 1207. ZEIN % x 6.25.
Solubility in Alcohol
Dissolve 1 gm in 10 ml of 80% alcohol.
Loss on Drying
Accurately weigh 2.0 gm of sample into a tared weighing dish. Dry at 105 degrees C for two hours. Cool to room temperature in a desiccator. Weigh and calculate the percent loss on drying as follows:
L.O.D. % = Wt. Sample Wet - Wt. Dry X 100
Wt. Sample Wet
Total Ash
Accurately weigh 2 gm of sample into a large tared crucible. Ignite the contents gently, then heat to a dull red. Cool to room temperature in a desiccator and add 1 ml concentrated sulfuric acid. Slowly heat to 800-850 degrees C. Hold at that temperature for two hours. Cool to room temperature in a desiccator. Weigh and calculate the total ash as follows:
Total Ash % = Sample wt. - Wt. residue X 100
Weight Sample
Heavy Metals
Proceed as directed under “Heavy Metals” (231) USP XXI, p. 1189 Method II
Arsenic
Proceed as directed under “Arsenic” (211) USP XXI, p. 1187
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